Thle2 cells
WebTThe THLE-2 ( ATCC CRL-2706 and the THLE-3 (ATCC CRL-11233) cell lines were derived from primary normal liver cells by infection with SV40 large T antigen. [RF84749] The virus was generated by introducing a retroviral vector containing the of Bgl I-Hpa I fragment of … Certain materials in the collection may require one or more permits to be filled ou… See all new products See what's coming next. ATCC (American Type Culture Colle… WebThe wild type and oncogene-transformed THLE2 cell lines were inoculated subcutaneously in nude mice (approximately 5×10 5 cells per animal, with 5 nude mice in each group). The tumor tissues were dissected, and the tumor sizes were …
Thle2 cells
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WebProgeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.The cured cell line is available as CRL-2706. The original patent deposit is available as CRL-10149. WebDec 29, 2024 · LAMC1 expression was higher in HCCLM3 and Huh7 cells than in THLE2 cells (Figure 5(e)). To determine whether miR-1343-3p interferes with LAMC1 expression, we transfected the miR-1343-3p inhibitor, si-LAMC1, or NCs into HCC cells. Western blot analysis showed that the miR-1343-3p inhibitor rescued LAMC1 expression in HCC cells …
WebSep 9, 2015 · In the present study, we firstly verified this result by qRT-PCR. We found that the gene expression levels of miR-137 were lower in all 7 probed HCC cell lines, when compared with the expression level of miR-137 in THLE2, a normal human hepatocyte cell line (P<0.05, Fig. 1A). WebMay 15, 2024 · Similar results were also observed in different cell lines, including the normal human liver THLE2 cells (Fig 1E and 1F). It is known that an increase in the number of LC3 puncta can be a result of an increased autophagic activity or a blockage of the fusion of autophagosomes with lysosomes .
WebHCC cell lines and, to a lesser extent, in the immortalized cell lines, but no effects on apoptosis were seen (Figure 1A and B). In contrast, after 24 hours, dramatically increased acetylation occurred in all HCC cell lines. Moreover, ap-optosis was induced and proliferation was decreased (Fig-ure 1C–E). Notably, the HCC cells show a much ... WebIn vitro evaluation of cytotoxic, oxidative, genotoxic, and apoptotic activities of physodic acid from Pseudevernia furfuracea in HepG2 and THLE2 cells Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology 1 Kasım 2024
WebThe wild type and oncogene-transformed THLE2 cell lines were inoculated subcutaneously in nude mice (approximately 5×10 5 cells per animal, with 5 nude mice in each group). …
WebAug 1, 2024 · THLE2 cells were incubated with 50 μM, 100 μM or 500 μM of BDA; or 7.5 mM, 10 mM or 15 mM of glycidamide for 24 h. As glycidamide was dissolved in DMSO, a solvent control was also included. After culture medium removal, cells were washed with PBS 1x and detached by incubation with PBS 2 mM EDTA for 20 min. After ... early stage athlete\u0027s foot cureWebAug 1, 2016 · THLE2 cells were cultured in LHC-8 medium (Life Technologies) supplemented with 5 ng/ml EGF, 70 ng/ml phosphoethanolamine, 9.2 ng/ml retinoid acid, 10% FBS and 1% penicillin-streptomycin. All cell cultures were maintained at 37°C in a humidified incubator with 5% CO 2. csu frammersbachcsuf psyc mastersWebAug 15, 2024 · HepG2 human hepatocellular carcinoma cells and Thle2 human liver epithelial cells were untreated or treated with different concentrations of SIN (Figure 1A; 25, 50, 75, and 100 μM) for 24 h or 48 h, and cytotoxicity was measured by the MTT assay. SIN reduced the viability of HepG2 and Thle2 cells in a concentration- and time-dependent … early stage anxiety stress hives treatmentWebJun 29, 2024 · The fundamental cause of transcription dysregulation in hepatocellular carcinoma (HCC) remains elusive. To investigate the underlying mechanisms, comprehensive 3D-epigenomic analyses are performed in cellular models of THLE2 (a normal hepatocytes cell line) and HepG2 (a hepatocellular carcinoma cell line) using … early stage before and after eczema treatmentWebOct 13, 2024 · Abstract The aim of this study was to evaluate hepatic polyunsaturated fatty acids (PUFAs) and inflammatory response in an animal and cell model of endoplasmic reticulum (ER) stress. Rats were divided into control, tunicamycin (TM)-treated, and TM + tauroursodeoxycholic acid (TUDCA)-treated groups. Hepatic ER stress was induced by TM … early stage basal cell skin cancer treatmentWebOct 24, 2024 · Immortal hepatocyte cell lines are widely used to elucidate insulin-dependent signalling pathways and regulation of hepatic metabolism, although the often tumorigenic … csuf promotional items