WebAbstract Cell surface receptors, such as transferrin receptors and MHC molecules, are internalized into the endocytic pathway and recycled to the plasma membrane. Previous assays used to measure endocytosis and recycling were cumbersome and often required radioactive reagents. WebAn optimized biotin-to-probe ratio can greatly increase the signal output of a detection system making it possible to create very sensitive assays. Biotin-labeled antibodies and other molecules are readily available from commercial suppliers making assay development routine for many applications.

Biotinylation and Haptenylation Reagents—Section 4.2

WebSep 21, 2024 · Furthermore, we performed surface biotinylation assay to examine the plasma membrane content of CD36 and ATP1A1. As Triton X-100 used in the assay would not disrupt the caveolae structures,... WebBiotin quantitation kits are used for determining biotinylation levels and efficiency by either colorimetric or fluorescence detection methods. These kits contain the simple and accurate HABA dye used to measure the amount of biotin attached to proteins or other biomolecules. Individual fluorescent quantitation reagents are also available for ... chicken futures

Monitoring MHC-II Endocytosis and Recycling Using Cell …

WebBiotinylation reagents for the study of cell surface proteins. The extraordinarily stable, non-covalent interaction between avidin and biotin is one of the most commonly exploited … WebThe OCW Assay can be used to: Detect and quantify target proteins localized to the cell surface; Quantify ligand binding to cell surface receptors; Monitor receptor internalization and recycling by detecting loss … WebOct 9, 2024 · Surface and endocytic EGFR were localized by biotinylation assay. 59 For the surface EGFR, the cells were biotinylated with 0.5 mg/ml NHS-SS-biotin for 30 min at 4 °C, washed with ice-cold ... chicken fusilli with spinach \u0026 asiago cheese