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Phellsgate 12

Webbecause of the extensive use of Verticillium race 1-resistant culti-vars (Dobinson et al., 1996). Currently, no source of commercially employed resistance to Verticillium race 2 strains has been WebAlternatively, generic gene-silencing vectors such as the pHANNIBAL and the pHELLSGATE series can be used. They simply require the insertion of PCR products, derived from the target gene, into the vectors by conventional cloning or by using the Gateway directed recombination system.

Constructs and methods for high-throughput gene silencing in plants

WebpHELLSGATE 12. High-throughput binary Gateway® destination vector with two introns, for silencing genes in plants using intron-containing hairpin RNA. Open in SnapGene. Try … WebSep 14, 2024 · For Sl-AGL11 down-regulation, a construct enabling RNAi silencing was designed using the pHELLSGATE-12 system (Invitrogen). The Sl-AGL11 3ʹ end was … pre cut weed trimmer line https://round1creative.com

Constructs and Methods for Hairpin RNA-Mediated Gene

WebAug 1, 2003 · The pHELLSGATE vectors contain convenient restriction sites that can be used to check for the presence of inserted sequences and diagnose the orientation of the … WebNov 23, 2011 · A schematic map of the Gateway cloning system-based RNAi vector, pSGate1. The ccdB cassettes were derived from pHellsgate-12 ( 24) and cloned into the silencing vector pSilent1 ( 9 ). Unique restriction sites are indicated. WebNational Center for Biotechnology Information scorchers fixtures 2020

pHELLSGATE 12 · Benchling

Category:A high-throughput inducible RNAi vector for plants

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Phellsgate 12

MutS-Homolog2 silencing generates tetraploid meiocytes in

WebpHELLSGATE 12 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available … WebDan Hernandez October 12, 2011. There's a Bank of America ATM just outside the gate. 13 Photos. Related Searches. gate a12 philadelphia • gate a12 philadelphia photos • gate a12 …

Phellsgate 12

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WebApr 5, 2024 · [0072] 实施例4：mswrky41基因转化紫花苜蓿的获得 [0073] 农杆菌准备：将在-80℃冰箱中保存的phb-mswrky41-flag和phellsgate-mswrky41rnai农杆菌菌株分别在含有抗生素为kan50+rif100和spe50+rif100的yeb固体培养基上划线活化，28℃培养48h后，挑取单克隆至含有相同抗生素的yeb液体培养 ... WebOct 1, 2002 · The destination vector was pHellsgate12 (CSIRO Plant Industry, Canberra, Australia), a Gateway® adapted binary vector suitable for gene silencing in plants (Helliwell et al. 2002).

WebFeb 5, 2024 · Nearby homes similar to 11412 Plenty Gates Ct have recently sold between $495K to $620K at an average of $170 per square foot. SOLD BY REDFIN JUN 10, 2024. … WebDec 10, 2024 · The plasmid pHellsgate 12:: Bngdsl1 was transformed into A. tumefaciens strain GV3101. The plants were then grown in a protected field in Zhenjiang, China, and transformed by Agrobacterium -mediated transformation according to the procedure described by Wang et al. ( 2003 ).

WebJan 4, 2024 · The resulting vector pHellsgate 12:: BnMAN7A07- RNAi contains a neomycin phosphotransferase II (NPT II) gene in its T-DNA region for the selection of transgenic plants by kanamycin. Agrobacterium tumefaciens strain GV3101 harbouring the plasmid pHellsgate 12:: BnMAN7A07- RNAi was used for transformation. WebOct 1, 2010 · A silencing vector (pSGate1) based on the Gateway cloning system was developed and used to construct RNA interference (RNAi) vectors. Silencing of GFP and ToxA in the transformants was demonstrated by transformation with the RNAi construct expressing hairpin RNA (hpRNA) of the target gene.

WebpHELLSGATE, pWATERGATE, pSTARGATE and pOpOff contain Gateway TM recombination sites. Gateway TM is proprietary technology owned by Invitrogen and to the extent that CSIRO vectors incorporate Gateway TM technology, CSIRO only has permission to distribute these vectors to educational and research organisations for research purposes.

WebDec 23, 2001 · The pHELLSGATE vector was tested using 200 and 400 nt PCR products for two different genes. Many bacterial colonies were obtained on chloramphenicol … scorchers final optusWebThe destination vector was pHellsgate12 (CSIRO Plant Industry, Canberra, Australia), a Gateway® adapted binary vector suitable for gene silencing in plants (Helliwell et al. 2002). precut white wood shelvingWebhomology to any other tomato gene was amplified and cloned into pHELLSGATE 12 vector (Figure S2). A total of ten independent transgenic lines bearing MSH2-RNAi construct were raised using Agrobacterium-mediated transformation. Southern blot analysis of T 0 lines revealed the presence of the transgene infive lines (Figure S3). In T scorchers fixtures 2021WebDec 1, 2024 · The study has also demonstrated the application of a hairpin construct using the pHellsgate 12 vector as an effective method to silence AYVMV. The viral amplicon encompassing overlapping regions of C1 , C2 and C3 (position 1234–1574 indicated on Fig. 1 a) genes was transferred to tomato ( Solanum lycopersicum ) (cv. MT1) through … scorchers games perthWebMay 31, 2012 · For each transformation, 12 clones (when less than 12, pick all) were identified by PCR using vector specific primes P21 and P22, and insert reverse primer P12 ( Fig. S1, Table S1 ), which can amplify the two arms simultaneously with a … scorchers game todayWebselection markers in the pHELLSGATE 12 vector using Xho1 and Xba1 adapter sequences as described in “Experimental procedures”. The RNAi construct (pVe2N2) was cloned in Agrobacterium, strain EHA105, which was then used to transform resistant (Cr) and suscep-tible (Cs) tomato near-isolines cv. Craigella based on procedures by scorchers grand finalWebFrontiers in Plant Science www.frontiersin.org 1 May2024 Volume 12 Article 672232. fpls-12-672232 May 13, 2024 Time: 20:35 # 2 ... (277 bp) of SlTPL1 into the pHellsgate 12 vector. The primers scorchers gear