Nettet30. mai 2024 · The Lineweaver–Burk plot (or double reciprocal plot) is a graphical representation of the Lineweaver–Burk equation of enzyme kinetics, described … The Lineweaver-Burk plot derives from a transformation of the Michaelis-Menten equation , in which the rate is a function of the substrate concentration and two parameters , the limiting rate, and , the Michaelis constant. Taking reciprocals of both sides of this equation it becomes as follows: Se mer In biochemistry, the Lineweaver–Burk plot (or double reciprocal plot) is a graphical representation of the Lineweaver–Burk equation of enzyme kinetics, described by Hans Lineweaver and Dean Burk in 1934. The Lineweaver–Burk … Se mer $${\displaystyle [S]}$$: substrate concentration. The independent axis of a Lineweaver-Burk plot is the reciprocal of substrate concentration, $${\displaystyle {\frac {1}{[S]}}}$$. $${\displaystyle V}$$ or $${\displaystyle V_{0}}$$: … Se mer When used for determining the type of enzyme inhibition, the Lineweaver–Burk plot can distinguish competitive, pure non-competitive Se mer • Michaelis–Menten kinetics • Eadie–Hofstee diagram • Hanes–Woolf plot Se mer The plot provides a very useful graphical method for analysis of the Michaelis–Menten equation, as it is difficult to determine precisely the Vmax of an enzyme-catalysed reaction: $${\displaystyle V={\frac {V_{\max }[S]}{K_{m}+[S]}}}$$ Se mer While the Lineweaver-Burk is useful for determining important variables in enzyme kinetics, it is prone to error. The y-axis of the plot takes the reciprocal of the rate of reaction, meaning small errors in measurement are more noticeable. Additionally, the … Se mer • NIH guide, enzyme assay development and analysis Se mer
How to calculate Km and Vmax values - Lineweaver Burk plot in …
NettetFitting Lineweaver-Burk Plot. As we know, the model parameters can also be estimated by the Lineweaver–Burk or double-reciprocal plot. ... (You can also use the Fit Linear … NettetTypes of inhibitors according to lineweaver-burk plot. Lineweaver–Burk plots of (a) competitive inhibition; (b) uncompetitive inhibition; (c) noncompetitive inhibition. 2. sino … healthy appetite
4.10: Lineweaver-Burk Plots - Biology LibreTexts
Nettet3. nov. 2006 · The initial velocity (v o) of the reaction, in micromoles of product produced/minute, can be calculated from the increase in absorbance at 400 nm (ΔA 400) measured at timed intervals during the reaction.Typical reaction mixtures to which the enzyme is added are shown in Table I.These mixtures provide a satisfactory … Nettet5. mar. 2024 · Lineweaver-Burk [ 4] (LB) plot, still the most popular and favored plot amongst the researchers, is defined by an equation: The -intercept in this plot is , the -intercept in second quadrant represents , and the slope of the line is . Eadie-Hofstee [ 5] (EH) plot is a semireciprocal plot of versus . NettetLineweaver–Burk plots for free and immobilised β -galactosidase. Enzyme concentrations within the gel are: 0.10 mg ml −1 ( ); 0.17 mg ml −1 ( ); and 0.50 mg ml −1 ( ). Data … healthy appetite with ellie krieger