2024 Cyclin e he12

Cyclin e he12

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August undefined, 2024

WebCyclin E1 (HE12) Mouse mAb detects endogenous levels of total cyclin E1 protein. It does not cross react with cyclin E2. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human cyclin E1. Background Cyclin E1 and cyclin E2 can associate with and activate CDK2 (1). Webcyclin A (H432) and cyclin E (HE12 and HE111 for immunoblot-ting and immunoprecipitation, respectively) were from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-phospho-CDK2 (Thr160) and ... The HA-cyclin E expression plasmid was constructed by sub-cloning the full-length cyclin E into pcDNA3.1 vector, and is expressed as an N …

Cyclin E1 (HE12) Mouse mAb from Cell Signaling Technology

http://www.ybiotech.com.cn/ybiotech2012-Products-18645796/ WebAnti-Cyclin E Antibody, clone HE12 is a high quality Mouse Monoclonal Antibody for the detection of Cyclin E & has been validated in IP, WB, ICC. Quality routinely evaluated in … new farm pond

cyclin E Antibody (HE12) SCBT - Santa Cruz Biotechnology

Web上海易汇生物科技有限公司，是一家集研发、销售为一体的高新技术生物企业，公司专注于生命科学和生物技术领域，专业提供分子生物学、免疫学、生命科学基础研究以及临床检测等诸多领域的试剂、耗材、仪器等各类产品及生物技术服务。公司目前已授权代理 LKT Laboratories，Epigent WebMCM7 (141.2), anti-cyclin E (HE12), anti-cyclin A (H-432), anti-cyclin B (H-433), and anti-CDK2 (M2) antibodies were purchased from Santa Cruz Biotechnology. Anti-ORC2 and anti- -actin antibodies were pur-chased from BD Biosciences and Sigma, respectively. Anti-p53 antibody was obtained from Cell signaling technology. Anti-pRB antibody was WebCyclin E Antibody (HE12) Product Data Sheet Product Specific Information General Information MA5-14336 targets Cyclin E in FACS, IF, IHC (P), and WB applications ... Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only new farm post shop

Detection of Low Molecular Weight Derivatives of Cyclin E1 Is a ...

Cyclin E Monoclonal Antibody (HE12), Biotin (13-9714-82)

WebHE12: Immunogen : A synthetic peptide of human Cyclin E Conjugate: Unconjugated : Form: Liquid: Concentration: 0.5 mg/mL: Purification: Protein A: Storage buffer: PBS with … WebSep 24, 2024 · Instead, increased expression of the full-length form of cyclin E1 (nucleus localized) was detected by western blotting using the monoclonal anti-cyclin E antibody (clone HE12), which was... new farm populationWebThis antibody recognizes a doublet of 50 kDa and a single band of 42 kDa identified as cyclin E. Cyclin proteins are a family of related proteins, which control the progression of the eukaryotic cell cycle. Overexpression of cyclin E shortens the length of the G1 phase, accelerating progression of S phase. The activity of cyclin E is mediated through its … intersection of poverty and trauma

"WebJul 6, 2004 · Cyclin E protein is overexpressed on a per cell basis in SUM149PT cells. (a) HE12 cyclin E antibody was used to detect cyclin E by immunoblotting MCF10A and SUM149PT whole cell lysates.(b) One-parameter flow cytometric analysis was performed on fixed and permeabilized cells using HE12 cyclin E primary antibody and FITC secondary … " - Cyclin e he12

Cyclin e he12

Distinct roles for cyclins E and A during DNA replication complex ...

WebJul 18, 2006 · Abstract. Cyclin E1 regulates the initiation of the S phase program in the mammalian cell division cycle. In normal cells, cyclin E1 protein expression is tightly controlled through a combination of transcriptional and proteolytic regulatory processes. However, in many types of human tumor, cyclin E1 expression is frequently … WebJul 6, 2004 · HE12 cyclin E primary monoclonal antibody and FITC-conjugated secondary antibody were used for cyclin E detection. Cells were incubated with 50 μg/ml propidium iodide (PI) for DNA content analysis. The results revealed that ~38% of cycling MCF10A cells were positive for cyclin E protein.

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WebCompany: Santa Cruz Antibody: cyclin E Catalog number: sc-247 Summary: Mouse monoclonal antibody against recombinant human cyclin E. Recommended for detection … WebCyclin E1 (HE12) Mouse mAb: Western Blotting; H Mk: ... Cyclin E/CDK2 further phosphorylates Rb to allow progression into S-phase, where cyclin E/CDK2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis (2,3). Turnover of cyclin E is largely controlled by phosphorylation that results in SCFFbw7-mediated ...

Webcyclin E Antibody (HE12) is an IgG1 κ mouse monoclonal cyclin E antibody suitable for the detection of cyclin E (doublet at 50 kDa and single band at 42 kDa) of mouse, rat and … WebAug 1, 1998 · Cyclin E and the cyclin-dependent kinase inhibitor p27 are two important regulators of the G1-S transition modulating the activity of cyclin-dependent ... Only the nuclear staining was evaluated because of earlier reports suggesting that the HE12 antibody can react with unrelated cytoplasmic antigens22 and a median fraction of 5.3% cyclin E ...

WebInvitrogen Anti-Cyclin E Monoclonal (HE12), Catalog # 32-1600. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (IHC) and … WebOct 7, 2024 · Previous experiments demonstrated that cyclin D-CDK4/6 and cyclin E/A-CDK2 induce Rb phosphorylation 9,13,17,18,19,20. However, the timing of Rb/E2F …

WebProduct Specific Information. Description: The monoclonal antibody HE12 recognizes human Cyclin E. Cyclins regulate the cell cycle by binding and activating cyclin … intersection of privacy and securityWebNO donor was associated with the down-regulation of cyclin D1 and hypophosphorylation of the retinoblastoma protein. No changes in the levels of cyclin E or the catalytic partners of these cyclins, CDK2, CDK4, or CDK6, were observed. This NO-induced cytostasis and decrease in cyclin D1 was reversible for up to 48 h of DETA-NONOate (1 mM) treatment. intersection of race and povertyWebAnti-Cyclin E Antibody, clone HE12 Antibody, human (mouse monoclonal IgG) Anti-Cyclin E Antibody, clone HE12 is a high quality Mouse Monoclonal Antibody for the detection of Cyclin E & has been validated in IP, WB, ICC. - Find MSDS or SDS, a COA, data sheets and more information. new farm postcode brisbaneWebCyclin E binds and activates the kinase Cdk2 and by phosphorylating its substrates, the so-called "pocket proteins", the cyclic/Cdk2 complexes initiate a cascade of events that leads to the expression of S-phase specific genes. Aside from this specific function as a regulator of S-phase-entry, cyclin E plays a direct role in the initiation of ... intersection of race and genderWebInvitrogen Anti-Cyclin E Monoclonal (HE12), Catalog # MA5-14336. Tested in Western Blot (WB), Immunohistochemistry (Paraffin) (IHC (P)) and Flow Cytometry (Flow) applications. This antibody reacts with Human, Mouse … intersection of row and column is calledWebSep 11, 2024 · We also observed that the Cyclin E antibody utilized here, the widely used clone HE12, detects a strong nonspecific signal in MCF10A cells, in addition to detecting Cyclin E (S6A and S6B Fig). Thus the difference in Cyclin E signal between CDK2 low and CDK2 inc cells may be partly obscured by the nonspecific signal. new farm police stationWebcyclin D1 rabbit polyclonal antibody (sc-753), mouse monoclonal anticyclin E (HE12) and cyclin A (BF683), goat polyclonal anti-p27Kip1 (C19), and rabbit polyclonal anti-Skp2 (H435) (Santa Cruz Biotechnology). Histone kinase assays were per-formed as described (9, 17), using rabbit polyclonal antibodies to Cdk2 (M2) (Santa Cruz Biotechnology). new farm powerhouse