Click-it edu imaging kit invitrogen
WebMar 9, 2015 · ®1.5 To make a 10X stock solution of the Click-iT EdU buffer additive (Component G), add 2 mL of deionized water to the vial and mix until the Click-iT® EdU buffer additive is fully dissolved. After use, store any remaining stock solution at ≤–20˚C. When stored as directed, the stock solution is stable for up to 1 year. WebDesigned to label and detect incorporated EdU and perform cell cycle analysis on samples from adherent cells. Manufacturer: Invitrogen™ C10640. Catalog No. C10640. $764.00 / Each of 1. Qty Check Availability. Add to cart. Includes: EdU (5-ethynyl-2' -deoxyuridine), Alexa Fluor dye-picolyl azide, anhydrous dimethylsulfoxide (DMSO), Click-iT ...
Click-it edu imaging kit invitrogen
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WebClick-iT EdU 细胞增殖成像试剂盒是一种优于传统细胞增殖检测的替代方法，其针对荧光 … WebClick-iT® Plus EdU Flow Cytometry Assay Kits, 100 tests Catalog numbers C10633, …
WebThe Click-iT® EdU Alexa Fluor® 488 HCS Assay is a superior alternative to traditional proliferation assays that is optimized for high-content imaging applications. In this assay the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor® dye in a ... WebSuperior Proliferation Methodology The Click-iT EdU Alexa Fluor 555 Imaging Kit provides a superior alternative to BrdU assays for measuring …
Webmethod. In contrast, the Click-iT® Plus EdU cell proliferation kit is compatible with cell cycle dyes, R-PE, R-PE tandems, and fluorescent proteins such as GFP, RFP, and mCherry. Figure 2 below demonstrates Click-iT® Plus EdU multiplexed with Hu CD3 PE and Figure 3 (page 3) shows Click-iT® Plus EdU multiplexed with FxCycle™ violet and GFP. WebApr 11, 2024 · They were then transferred to standard medium for a 3h EdU-free chase. Larvae were dissected, fixed and processed for antibody staining, followed by EdU detection with Click-iT Plus EdU Cell Proliferation Kit for imaging, Alexa Fluor 647 dye (Invitrogen, #C10640) according to the manufacturer's instruction. Imaging and image processing
WebProduct overview. EdU Assay / EdU Staining Proliferation Kit (iFluor 488) ab219801 provides a sensitive and robust method to detect and quantify cell proliferation in live mammalian cells using flow cytometry or fluorescence microscopy. The iFluor 488 dye (Ex/Em: 491/520 nm) has spectral properties almost identical to those of FITC and ...
WebClick-iT® Plus EdU Imaging Kits 6 Detect EdU Note: This protocol uses 500 µL of Click-iT® Plus reaction cocktail per coverslip. A smaller volume can be used as long as the remaining reaction components are maintained at the same ratios. 4.1Prepare 1X Click-iT® EdU buffer additive (see Table 3, below) by diluting the 10X solution (prepared in step … dr javier ugarte roaWebMake 1X Click-iT® EdU reaction buffer by transferring the solution (4 mL) in the … dr javier rosa reumatologoWebMar 9, 2015 · Click-iT® EdU Imaging Kits . Table 1. Contents and storage information. … ramiro\u0027sWebFeb 13, 2024 · This experimental protocol is appropriate when the Click-It EdU Imaging Kit (Invitrogen) is used. To prepare a 10 mM EdU stock solution, an aqueous solution (i.e., buffer or saline) can also be used. After use, the stock solution can be stored at ≤ −20 °C and is stable for up to 1 year. The Click-It EdU Imaging Kit (Invitrogen) is ... dr javza natsagWebwahoo.nsm.umass.edu dr javorsWebMay 17, 2024 · After de-waxing, paraffin sections were incubated with the Click-iT EdU Imaging Kit (Invitrogen, CA, USA). SEM. Tongue samples were fixed in 4% PFA overnight and dehydrated in a graded series of ethanol concentrations. Dehydrated samples were then incubated in 50% hexamethyldisilazane (Sigma-Aldrich, St. Louis, USA) for 20 min, … dr javier roman cirujano plasticoWebAug 5, 2013 · EdU labeling and cell proliferation analysis was performed using Click-iT® EdU Imaging Kit with Alexa Fluor® (Life technologies), following the instructions of the manufacturer. 1.5×10 4 human UCs at passage 4 were seeded per well in 24-well plates. EdU was added to cells 12 h after seeding and pulsed for 2.5 h. The EdU-treated cells … dr. javvaji muskogee ok