WebNov 18, 2024 · ( A) BrdU labeling of mtDNA. Cells were incubated for 2 h in the absence or presence of oligomycin, as indicated. mtDNA was Southern-blotted and probed with anti-BrdU antibody (upper panel). In parallel, mtDNA was labeled with EtBr (lower panel). The specific activity of the BrdU-labeled DNA (BrdU intensity/EtBr density) is indicated. WebAssessment of newly synthesized mitochondrial DNA using BrdU labeling in primary neurons from Alzheimer's disease mice: Implications for impaired mitochondrial biogenesis and synaptic damage The purpose of our study was to assess mitochondrial biogenesis and distribution in murine primary neurons.

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WebMar 23, 2024 · Mitochondrial DNA and VDR co-localization. The co-localization of VDR with mitochondrial DNA (mtDNA) was determined by 5-Bromo-2-deoxy-Uridine (BrdU) labeling in untreated primary neurons and SH-SY5Y cells. BrdU is a thymidine analog and joins DNA while it is being synthesized. In this way, it enables the marking of newly … WebBrdU- or EdU-labeled mtDNA was identified and measured by the following protocol: fill holes in objects, separate touching objects by 0.1 mm3, and exclude objects by size … black box ideas

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Primary skin fibroblasts treated acutely with 10 mM 2DG received a 16 h pulse of 50 µM 5-bromo-2'-deoxyuridine (BrdU, Sigma). Parallel conditions without BrdU and/or without 2DG were used as controls. One microgram of isolated DNA was digested with ApoI restriction enzyme to generate ~300 bp fragments … See more A549 adenocarcinoma and MyoRD rhabdiomyosarcoma m.3243A>G cybrid cells5,11 were maintained in Dulbecco’s Modified Eagle’s … See more The cellular proliferation rate was determined using an IncuCyte Zoom cell imager (Essen Bioscience). In all, 3 × 104cells were seeded in 6-well plates and imaged every hour … See more Chemicals were purchased from Sigma, except for koningic acid (Abcam). Rotenone, oligomycin and 5-bromo-2′-deoxyuridine were … See more The release of the lactate dehydrogenase (LDH) in the medium was measured adapting the instructions from the manufacturer (Cytotoxicity Detection Kit, Roche). Briefly, … See more Web丁香通为您找到80条哈尔滨dna检测去哪里信息，包括哈尔滨dna检测去哪里报价行情，优质供应商，图片，品牌等最新信息，丁香通为买家提供用户服务，诚信保障等服务，批发采购哈尔滨dna检测去哪里,上丁香通。 WebMar 23, 2012 · Mitochondrial DNA directly induced NLRP3 inflammasome activation, because macrophages lacking mtDNA had severely attenuated IL-1β production, yet still underwent apoptosis. Both binding of oxidized mtDNA to the NLRP3 inflammasome and IL-1β secretion could be competitively inhibited by the oxidized nucleoside 8-OH-dG. galeton repel rainwear