2024 Ai9 tdtomato reporter mice

Ai9 tdtomato reporter mice

Author: lurv

August undefined, 2024

WebStudies in our own laboratory using the Ai9 TdTomato reporter line have shown Dmp1-Cre expression in some osteoblasts, skeletal muscle, a subset of cells in the marrow, stomach, small intestine, skin, brain, eye and an even more restricted subset of cells in the lung and kidney (see Fig 2). WebApr 10, 2024 · Ad5-knob(RGD/PK7) produce tdTomato signal in the hippocampus of Ai9 (loxP-tdTomato) mice. Fluorescence was abolished when the spike tip protein (Pvc10) was deleted, confirming that the observed activity was mediated by the ... For assays involving co-transfection of a Cre reporter plasmid or a guide. RNA plasmid, DNA was transfected …

Not All That Is Gold Glitters: PV-IRES-Cre Mouse Line Shows …

WebMar 7, 2024 · Strategic gating of unlabeled vasculature derived cells from WT mice enriches for SMCs. Further fluorescence activated cell sorting (FACS) analysis of tdTomato (tdT) sorted cells from Cspg4-Cre;Ai9-tdT mice described in Fig. 1 revealed that selectively gating for high side and forward scatter events (A) enriched for the tdT + smooth muscle cell … WebJun 22, 2024 · All mice carry the Ai9 TdTomato reporter. Inset shows a higher power view of TdTomato positive areas in brain. Bars = 200 μm. d Lung and kidney showed a low level of TdTomato reporter expression in 10-kb Dmp1-Cre (+) mice compared to Cre (−) littermates. The expression was seen in only a small subset of cells within the tissue. htit f heccr zp 9

tdTomato转基因小鼠的建系及其在细胞示踪方面的应用研究

WebThe ratio of the reporter to the reporter control in ESCC mice was also significantly higher than in healthy control mice (P<0.0001; Figure 5C and G). Moreover, the ROC curve … Web007909 B6.Cg- Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J Ai9 is a Cre reporter tool strain designed to have a loxP -flanked STOP cassette preventing transcription of a CAG … WebDec 31, 2015 · As expected, injection of AAV- Dmd CRISPR did not induce tdTomato expression in satellite cells or myofibers of mdx;Ai9 mice (fig. S11). In summary, this study provides proof-of-concept evidence supporting the efficacy of in vivo genome editing to correct disruptive mutations in DMD in a relevant dystrophic mouse model. hockey skates madison wi

Ai9 tdtomato reporter mice

Mouse Cre Models for the Study of Bone Diseases SpringerLink

WebHere, we present a novel reporter mouse model targeting a microglia-specific marker, TMEM119, for studying microglia in health and disease. By placing a reporter cassette (GSG-3xFlag-P2A-tdTomato) between the coding sequence of exon 2 and 3'UTR of the Tmem119 gene using CRISPR/Cas9 technology, we generated a Tmem119-tdTomato … WebJan 1, 2024 · Here, we examined the distribution of Cre and UCP1 throughout the body in UCP1-Cre reporter mice. Methods UCP1-Cre mice crossed to Ai14-tdTomato and Ai9-tdTomato reporter mice were used to explore the tissue distribution of Cre recombinase and Ucp1 mRNA in various tissues.

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WebApr 11, 2024 · We therefore examined sections from brain and spinal cord of NPFF Cre mice crossed with the Ai9 reporter line, in which Cre-dependent excision of a STOP cassette results in expression of... WebNov 8, 2024 · We used immunofluorescence for PV on tissue from mice expressing tdTomato in PV-INs to address the specificity and efficacy of the PV-IRES-Cre mouse line in the mouse PER. ... 743 tdTomato-expressing neurons, and 719 double labeled neurons. Similar to our results with the Ai9 reporter line, the specificity was very high (96.85 ± 1%, …

WebDec 27, 2024 · Given that low transduction efficiency of these serotypes by intravitreal injection 19, we delivered them to the retinas of Ai9 mice by subretinal injection (Fig. 1 A). We observed distinct... WebJan 5, 2024 · The Ai9 reporter mice harbors a lox-STOP-lox (LSL)-tdTomato reporter knockin at the Rosa26 locus 11 (Figure 1). Using two sgRNAs flanking LSL, Cas9 will delete the STOP cassette by NHEJ, resulting in tdTomato expression. 12 This system allows direct detection of cells edited by CRISPR in vivo.

WebPlasmid pDEST-Cas9-tdTomato from Dr. Hideyuki Okano's lab contains the insert Cas9 and is published in PLoS One. 2024 Aug 27;14(8):e0221164. doi: … Web用于例如CNS、PNS、内耳、心脏、或视网膜中的转基因表达的工程化AAV载体及其使用方法。还提供了开发在期望的细胞类型中介导转基因表达的新工程化AAV载体的方法。

WebOct 27, 2024 · Expression of the Cre-recombinase activity was evaluated using the reporter mouse strain Ai9 at pre-hearing, hearing onset, and post-hearing stages. Accordingly, heterozygous Nefl CreER/ + ;iDTR +/- mice were treated with tamoxifen on postnatal days 1-5 (P1-5), followed by diphtheria toxin (DT) or vehicle injection on P7, P14, and P21 to ... hockey skates on a hookWebApr 14, 2024 · The lack of GzmB in memory CD4 + T cell populations could result either from loss of GzmB expression over time within memory CD4 + T cells, or the specific loss of GzmB + CD4 + T cells themselves. To differentiate between the two possibilities, we rechallenged Ad5-pIX-gp70 or BCG.SOD-env 122-141 immunized mice using different … hti thermalölWebNov 3, 2024 · With the application of tdTomato reporter, acute brain slices were prepared, and patch-clamp recordings were performed on Tac1 + neurons in the NAc lateral shell. We observed a decreased excitability of Tac1 neurons in susceptible mice following CSDS, whereas resilient animals exhibited excitability levels similar to controls ( Figures 2 D and … hockey skates loose or tightWebDec 20, 2009 · Strong tdTomato native fluorescence in Ai9 and Ai14 allowed direct visualization of fine structural details, such as dendritic spines and thin axons, of diverse … hti thermalWebThis was achieved by crossing the HDC-Cre mouse line with Rosa26-tdTomato reporter mice, thus resulting in the expression of the fluorescent Tomato (Tmt) signal in cells … hockey skates play it again sportsWebNov 12, 2024 · C57BL/6 (wild type, WT) and Ai14/Rosa26-tdTomato Cre-reporter (B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J) male and female mice (6–8 weeks old) … hockey skates online adult size 15 ddWebAug 12, 2024 · Ai9 is a Cre reporter tool strain designed to have a loxP -flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt (ROSA)26Sor locus. Ai9 mice express robust tdTomato fluorescence following Cre-mediated recombination. hockey skates pronated feet